Name: NAD+ Spray | 1500mg | 30ml | Captide LabsResearch-grade NAD+ spray — 1500mg per 30ml, 7–8mg per actuation (~200 sprays), >98% purity (HPLC-MS verified). CAS 53-84-9. Cellular coenzyme for redox, sirtuin & longevity research. Third-party tested, RUO.
SKU: CPT-SPRY-NAD+-30ML
Price: 119.99 USD
Availability: InStock

Description

Metabolic →·Cognitive →

NAD+ Research Spray | Nicotinamide Adenine Dinucleotide & Sirtuin Coenzyme

Captide Labs NAD+ Spray delivers research-grade Nicotinamide Adenine Dinucleotide in a pre-formulated, high-concentration liquid spray format. Each 30ml bottle contains 1500mg of NAD+ at greater than 98% purity, independently verified by HPLC-MS analysis and documented with a full Certificate of Analysis. Each actuation delivers approximately 7–8mg, providing roughly 190–215 sprays per bottle.

NAD+ (CAS 53-84-9, also known as Nadide, Coenzyme I, or DPN+) is a naturally occurring dinucleotide coenzyme found in all living cells. It plays a fundamental role in cellular energy metabolism, redox biology, and DNA repair signaling — serving as the obligate substrate for sirtuin deacetylases (SIRT1–7), PARPs, and CD38, and as the principal electron carrier in mitochondrial oxidative phosphorylation. NAD+ levels decline significantly with age (research indicates up to a 50% reduction by middle age), making NAD+ repletion one of the most actively investigated interventions in longevity, metabolic, and neuroprotective research.

Research Specifications

Compound

NAD+ (Nicotinamide Adenine Dinucleotide)

CAS Number

53-84-9

Molecular Formula

C₂₁H₂₇N₇O₁₄P₂

Molecular Weight

663.43 g/mol

Form

β-NAD (free acid, oxidized)

Synonyms

Nadide, Coenzyme I, DPN+, β-NAD

Purity

>98% (HPLC-MS Verified)

Format

Liquid Spray

Concentration

50mg/ml

Bottle Volume

30ml

Total Content

1500mg

Spray Delivery

7–8mg per actuation (~200 sprays)

Testing Standard

ISO 17025 Accredited Lab

Storage

Refrigerate at 2–8°C. Protect from light. Use within 28–30 days of opening.

What is NAD+?

Nicotinamide Adenine Dinucleotide (NAD+) is a dinucleotide coenzyme present in all living cells and essential to hundreds of metabolic reactions. It functions as an electron carrier in oxidation-reduction (redox) reactions — cycling between its oxidized form (NAD+) and reduced form (NADH) — making it a central mediator of cellular energy production through glycolysis, the citric acid cycle, and oxidative phosphorylation. The standard midpoint potential of the NAD+/NADH redox pair is −0.32 V, characterizing NADH as a moderately strong reducing agent.

Beyond its role in energy metabolism, NAD+ is a required substrate for several classes of regulatory enzymes including sirtuins (SIRT1–7), PARPs (poly ADP-ribose polymerases), and CD38 — proteins with key roles in DNA repair, gene expression regulation, circadian clock function, and cellular stress responses. Research interest in NAD+ has expanded dramatically in the context of aging biology, where declining NAD+ levels correlate with reduced mitochondrial function, impaired DNA repair, and age-related metabolic dysfunction.

Research Overview

Areas of Active NAD+ Investigation

Sirtuin Activation & Longevity Signaling

Sirtuins are NAD+-dependent deacetylases that regulate gene expression, mitochondrial biogenesis, stress resistance, and cellular aging pathways. SIRT1 and SIRT3 in particular have been extensively studied for their roles in metabolic regulation and longevity signaling. Because sirtuin activity is directly dependent on NAD+ availability, researchers use NAD+ supplementation models to investigate how NAD+ levels influence sirtuin-mediated gene regulation, mitochondrial function, and age-associated cellular decline in controlled experimental systems.

DNA Repair & PARP Signaling

PARP enzymes consume NAD+ as a substrate during DNA strand break repair, using it to synthesize poly(ADP-ribose) chains that signal and recruit repair machinery to damage sites. Under conditions of high DNA damage, PARP hyperactivation can deplete cellular NAD+ pools, impairing energy metabolism and accelerating cell death. Researchers studying DNA repair pathways, genomic stability, and the relationship between oxidative stress and NAD+ depletion use exogenous NAD+ to examine how coenzyme availability modulates PARP activity and repair efficiency.

Mitochondrial Function & Cellular Energy Metabolism

NAD+ is indispensable to mitochondrial oxidative phosphorylation — the process by which cells generate the majority of their ATP. As an electron carrier in the electron transport chain, NADH donates electrons to Complex I, driving proton gradient formation and ATP synthesis. Research in aging models has consistently demonstrated that NAD+ decline correlates with reduced mitochondrial membrane potential, decreased ATP production, and impaired mitochondrial biogenesis. These findings have made NAD+ repletion a key area of investigation in metabolic disease and aging research.

Neuroprotection & Cognitive Research

The brain is among the most metabolically demanding tissues in the body and is particularly sensitive to NAD+ depletion. Preclinical studies have investigated NAD+ in models of neurodegeneration, traumatic brain injury, and ischemia, with findings suggesting neuroprotective effects mediated through SIRT1 activation, PARP regulation, and mitochondrial preservation. Intranasal delivery of NAD+ has attracted research interest as a route for bypassing first-pass metabolism and providing direct vascular delivery for systemic and CNS-targeted research applications.

Circadian Rhythm & Metabolic Regulation

NAD+ levels exhibit circadian oscillation, rising during active periods and declining during rest phases, reflecting its coupling to the cellular clock machinery. SIRT1 and CLOCK/BMAL1 form a feedback loop in which NAD+ availability influences circadian gene expression, and circadian gene activity in turn regulates NAD+ biosynthesis via NAMPT. Researchers studying the intersection of metabolism, aging, and circadian biology use NAD+ as a tool for investigating how coenzyme oscillation influences cellular clock function, metabolic timing, and the long-term consequences of circadian disruption.

NAD+ / NNMT Methylation Pathway Crosstalk

A growing area of research focuses on the interplay between NAD+ pools and the methylation enzyme NNMT, which consumes the NAD+ precursor nicotinamide. Researchers investigating how NNMT inhibition affects NAD+ availability — and conversely how NAD+ supplementation interacts with cellular methyl donor pools — frequently study NAD+ alongside NNMT inhibitors as a tool for characterizing both arms of the nicotinamide metabolic network in metabolic and aging research models.

Third-Party Certificate of Analysis

Every batch of NAD+ Spray is independently tested by an ISO 17025-accredited laboratory. View the full COA including identity confirmation, purity assay, heavy metals, and microbial results on the Captide Labs Lab Reports page.

View COA →

Frequently Asked Questions

What is NAD+ used for in research?

NAD+ is used across a broad range of research domains including aging biology, mitochondrial function, DNA repair, sirtuin signaling, neuroprotection, and circadian rhythm regulation. It is a required substrate for sirtuin deacetylases and PARP enzymes — two of the most studied protein families in longevity and genomic stability research — making it one of the most foundational compounds in contemporary cellular biology and metabolic research.

What is the concentration of this spray?

Each 30ml bottle contains 1500mg of NAD+, yielding a concentration of 50mg/ml. Each actuation delivers approximately 7–8mg, providing roughly 190–215 sprays per bottle. This high-concentration format is significantly more loaded than the 100–500mg vials typical in the research market, making it well-suited for protocols requiring repeated high-dose delivery over an extended period.

How does NAD+ differ from NMN and NR?

NMN (Nicotinamide Mononucleotide) and NR (Nicotinamide Riboside) are NAD+ precursors — compounds that are converted to NAD+ intracellularly via biosynthetic pathways. They are frequently used in oral supplementation research because they are more orally bioavailable than NAD+ itself, which is poorly absorbed across the gastrointestinal epithelium. NAD+ delivered intranasally bypasses this limitation, entering systemic circulation directly through the nasal mucosa’s vascular network without requiring biosynthetic conversion — providing direct coenzyme delivery rather than precursor-dependent synthesis.

What is the CAS number for NAD+?

The CAS number for NAD+ (Nicotinamide Adenine Dinucleotide, oxidized free acid form) is 53-84-9. Its molecular formula is C₂₁H₂₇N₇O₁₄P₂ with a molecular weight of 663.43 g/mol. It is also referenced under the synonyms β-NAD, Nadide, Coenzyme I, and Diphosphopyridine Nucleotide (DPN+). The reduced form NADH has a separate CAS number (58-68-4) and the phosphorylated form NADP+ is also distinct (CAS 53-59-8).

Why use a nasal spray format for NAD+?

NAD+ has poor oral bioavailability — it is largely degraded by gastrointestinal enzymes before absorption. IV administration achieves direct systemic delivery but requires clinical infrastructure. The intranasal route offers a practical middle ground: the nasal mucosa’s rich vascular network provides direct access to systemic circulation, bypassing hepatic first-pass metabolism and gastrointestinal degradation. For CNS-targeted research specifically, the nasal route also provides proximity to the olfactory nerve pathway, offering a potential direct access point to neural tissue.

How should NAD+ Spray be stored?

Store NAD+ Spray refrigerated at 2–8°C. NAD+ is sensitive to heat, light, and oxidation — all of which can accelerate degradation of the dinucleotide structure. Keep the bottle tightly capped and away from direct light at all times. Once opened, use within 28–30 days. Do not freeze once in liquid form. NAD+ in its solid (lyophilized) powder form is hygroscopic and is best preserved in airtight, light-protected storage at -20°C for long-term stability.

For research use only. Not for human consumption, veterinary use, or food/agricultural applications. Not evaluated or approved by the FDA. Not intended to diagnose, treat, cure, or prevent any condition. All purchases are made with the understanding that this compound is strictly for in-vitro research and laboratory use. See the full Research Use Only (RUO) Policy.

FDA Disclosure & Intended Use

FDA Disclosure: The statements on this website and the products sold herein have not been evaluated by the U.S. Food and Drug Administration (FDA). These products are not intended to diagnose, treat, cure, or prevent any disease. Products sold are for Research Use Only and are not for human or animal use.

Intended Purpose: Products sold on this site are intended solely for basic laboratory research, pharmaceutical research, or the development of new tests. They are not intended for diagnostic, therapeutic, or clinical use.

Storage Instructions

Handling and Storage Tips:

Keep peptides cold and away from light once received.
For short-term use (days to weeks), refrigeration at 4°C (39°F) is acceptable.
Lyophilized peptides are typically stable at room temperature for several weeks, making it suitable for moderate-term storage.
For long-term storage (months to years), it’s best to freeze peptides at -80°C (-112°F). Freezing optimally preserves peptide stability for extended periods.

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Research Use Only (RUO) Policy

"RUO" is an abbreviation for Research Use Only. It is a designation used primarily by the FDA (Food and Drug Administration) for products, often in vitro diagnostic (IVD) components like reagents or instruments, that are in the laboratory research phase of development and are not intended for clinical diagnostic use.

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